Table 3. Oligonucleotides used in this study.

Primer Name	Sequence (5′-3′)	Purpose
JP Afumi argB For	GAACGCGGTCTGCATCCAAG	Create pJMP4/argB
JP Afumi argB Rev	GAAGGAGAGACCCATACATCC	Create pJMP4/argB
JP Apara pyrG Short For	GCTTGATATCGAATTCTCATGTTTG	Create pJMP7
JP Apara pyrG Short Rev	TACGGAAGCTTATACGAACAGATGG	Create pJMP7
JP gpdA(p) EcoRI For	TTTCGAATTCCATCCGGATGTCGAAGG	Create pJMP8 and pRS1
JP gpdA(p) BamHI Rev	CTTGTGGATCCCGTCATCTTTGAAA	Create pJMP8
MK Afumi Bzip Fusion Rev	ATTGGAATAAAATGAGTAGTCCATGGTGATGTCTGCTCAAGCGG	Create pRS1
MK Afumi Bzip Fusion For	CCGCTTGAGCAGACATCACCATGGACTACTCATTTTATTCCAAT	Create pRS1
MK Afumi Bzip rev (EcoRI)	GTTGTACTAGAAGAGAATTCGCAGTC	Create pRS1
RS rsmA 5′F For	GTGAAGACGGCAATCTCTGAG	ΔrsmA 5′ flank
RS rsmA 5′ F+pyrg tail Rev	CCCTATAGTGAGTCGTATTACGATCTGTGCACTTCCCTCAAC	ΔrsmA 5′ flank
RS pyrG +3′F rsmA For	CGATGATAAGCTGTCAAACATGAGCCTTGGCAGGGCGAATTTGG	ΔrsmA 3′ Flank
RS rsmA 3′ F Rev	CAGCAAATCGTTCGTGCTCG	ΔrsmA 3′ Flank
RS rsmA screen For	GATCTACAACGCTTCGACGGAC	PCR screen rsmA
JP Af-laeA 5′ Fl For	CCTCGTTGAACTAATTGGCG	ΔlaeA 5′ Flank
JP Af-laeA 5′ Fl Rev	GGAAAATTTGTCTTGGATGCAGTCCGCGTTCAGGTCGAGGAGGTCCAATC	ΔlaeA 5′ Flank
JP Af-laeA 3′ Fl For	AGATCAAATGGATGTATGGGTCTCTCCTTCGCTTCAAACCTCTGAGATCG	ΔlaeA 3′ Flank
JP Af-laeA 3′ Fl Rev	CGACACACATATCATGACGG	ΔlaeA 3′ Flank
FY GZ 5′F FOR	GAGTTCTCGCTAGTGTCTGG	ΔgliZ 5′ Flank
RS ArgB tail +5′Rev	GTCTTGGATGCAGACCGCGTTCCGCAGTGCAACACGGGTGGC	ΔgliZ 5′ Flank
RS GliZ 3′ Fl For	CGCAGTGCAACACGGGTGGCGTCTTGGATGCAGACCGCGTTC	ΔgliZ 3′ Flank
FY GZ 3′F REV	CGAGTCAACATGATGACGG	ΔgliZ 3′ Flank
RS gliZ 5′ For	TGAGCCATACTGAAGTCTCCGTG	ΔgliZ nested
RS gliZ 3′ Rev	GACATCGACTGAACATGTCCACG	ΔgliZ nested
RS Af RsmA Int For	CAATCCTCAGTCACAGCAGC	rsmA probe
RS Af RsmA Int Rev	GGTCATATCAATTCATCGCGGC	rsmA probe
JP Modified T7 Promoter For	CGTAATACGACTCACTATAGGG	Amplify pyrG from pJMP7
RS A.para pyrG Rev	CTCATGTTTGACAGCTTATCATCG	Amplify pyrG from pJMP7
RS rsmA RT-PCR For	ACCGAGCTGCTCAGCGAGCG	rsmA RT-PCR
RS rsmA RT-PCR Rev	CTGGCCCTCCCTGAACGCCG	rsmA RT-PCR
RS actin RT-PCR For	GTCACCATGGTATCATGATTGG	actin RT-PCR
RS actin RT-PCR Rev	GGTAGTCCGTCAGATCACGG	actin RT-PCR
RS laeA RT-PCR For	CCAGTAGCAAGAATCCTGAC	laeA RT-PCR
RS laeA RT-PCR Rev	CCGGAGCCCATCTGCATGTG	laeA RT-PCR
RS gliZ int For	GCACCTACAGCTATTCCTCG	gliZ probe
RS gliZ int Rev	GTCACGGCCATGCTAATACTG	gliZ probe
RS actin int For	GTATGTCGGTGATGAGGCAC	Actin probe
RS actin int Rev	CCGTAGAGATCCTTACGGAC	Actin probe
GIINTF	TGTTGATCGAGACGCCGTTCTG	gliI probe
GIINTR	CAGAGCGGCTCGATTCTGGTG	gliI probe
RS gliT For	GCAAACTACTCTCCAACGGAG	gliT probe
RS gliT Rev	GCAAACTACTCTCCAACGGAG	gliT probe