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Lane 1, Protein Molecular Weight Marker (Fermentas); lane 2, soluble E. coli BL21 (DE3) [pET-23a(+)::aroK] extract; lane 3, Soluble proteins after 10 mM MgCl2 precipitation step; lane 4, Phenyl Sepharose 16/10; and lane 5, Sephacryl S-100 HR.
