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. 2013 May 6;8(5):e63741. doi: 10.1371/journal.pone.0063741

Table 5. Oligonucleotide primer sequences for real time PCR.

Gene Primer sequence Product size(bp)
ELOV F: 5′ ACGGTTCTCATCTATTGCT 3′ 149
R: 5′ ATCGCAATCAATTTCGGGAC 3′
Actin F: 5′ GAAGATCTGGCATCACACC 3′ 109
R: 5′ CATCTTCTCTCTGTTTGCCTT 3′
Cuticle protein 7 F: 5′ GAGGATCCAGCACACCAA 3′ 118
R: 5′ AACCGCATTTCCATATCCAC 3′
Cuticle protein 19 F: 5′ CCCTCAGCCATTTGCCTA 3′ 63
R: 5′ TTCTTGAAGTTAGCCTTAGAGT 3′
Ferritin F: 5′ AACCGTGATGATCAAGCTC 3′ 104
R: 5′ CGCTTGGTCTGATATTCCAT 3′
HSP 70 F: 5′ ATCTTCGAGGTCAAGTCCAC 3′ 116
R: 5′ TTCATGTCCTTCTTGTGCTT 3′
HSP 90 F: 5′ TGGTTTCTACTCCGCCTA 3′ 78
R: 5′ CAGACATATTGCTCGTCA 3′