Figure 2. Growth of P. gingivalis strains in the absence of NaCl.
Overnight cultures of P. gingivalis parent, rprY mutant (A), and complemented rprY mutant strains (B) were diluted 1∶40 (OD550 ∼0.02) in pre-reduced TSB media with or without NaCl and grown anerobically at 37°C. Parent strain with NaCl: black diamonds; rprY mutant with NaCl: black squares; parent without NaCl: black triangles; rprY mutant without NaCl: black circles; complemented rprY mutant with NaCl: open squares; complemented mutant without NaCl: open circles. The average of two independent experiments is shown. The experiment was repeated at least three times.