|
rprY-lacZ
cloning
|
|
|
rprY
|
ACCCGGGGATTTTTATTCCTGCCATAAC/GGGATCCTTACTATCGTCCTCGCAGAG (821) |
This study |
|
rprY
cloning in pET28a
|
|
|
his-rprY
|
AAGCTTGCATGCCTGCTCAGACCTCTTTGATCAACTC/ |
This Study |
|
TATACATATGGAAGAAAAAACAAGAATCTTTCTCT
|
|
|
rprY
cloning in pFD288
|
|
| rprYHis3 |
TAGACGTCTCAGTGGTGGTGGTGGTGGTGGACCTCTTTGATCAACTCCTCA
|
This study |
| KAR6a |
ACCCGGG
GAT TTT TATT CCT GCC ATA AC
|
This study |
|
OxyR deletion
|
|
| 3-way-oxyRF1 |
CGAGTGGCGATGAGCGTCAGGCC
|
This study |
| 3-way-oxyRR2 |
TTCCGAGCTTTCTTCTTTTGCCGT
|
This study |
| 3-way-oxyR R1-EM |
TGTAGATAAATTATTAGGTATACTACTGACAGCTTCGGGAAGGATCGTTTTGTATTAG
|
This study |
| 3-way-oxyR F1-EM |
ACCGATGAGCAAAAAAGCAATAGCGGAAGCTATCGGACGAATCATATTTCCTATTTTTG
|
This study |
|
RprY deletion
|
|
| KK108 |
GAGATAGAAGCATTAGAACTAATGGGCTACTAAAGGGCT
|
This study |
| KK109 |
GGAGATAATTCGTTGTTTATCCAGCTGAGAACGGAATGAG
|
This study |
| KK111 |
CTCATTCCGTTCTCAGCTGGATAAACAACGAATTATCTCC
|
This study |
| KK112 |
AGCCCTTTAGTAGCCCATTAGTTCTAATGCTTCTATCTC
|
This study |
| KK107 |
CTCGTGTTTATGGTTCCCAA
|
This study |
| KK110 |
GTCAGGGGAGGCTCTGCTTC
|
This study |
|
qPCR
|
|
|
| 16S |
TGTTACAATGGGAGGGACAAAGGG/TTACTAGCGAATCCAGCTTCACGG(118) |
[42]
|
|
sod
|
AAAGAGCGAAGGCGGTATCT/CGAATGAGCCGAATTGTTTGTC(140) |
[42]
|
|
ahpC
|
TCAAACTCAATGCCTATCACAATG/GATAGAAAACGACCAAAGACCACT(87) |
[42]
|
|
rprY
|
CCATCGCGATCGATGATCAGGTAA/GGCATAGTTGCGTTCAAGGGTTTC(104) |
[42]
|
| PG0275 |
ATCGGCAGATTGCCCATCGT/ATGCCGGTAAACTCACCGTCT(122) |
This study |
| PG1286 |
CAAGGCCGAAATGTGGTCTTCA/TCCATCATATCGTAGGCGTGCT(134) |
This study |
| PG1134 |
CTCATCATCGGTTCCGGACCT/TTCCACCTCGGTCGTAGTCGT(125) |
This study |
| PG2008 |
GGATCCGTTACAGGTACCGTAGT/ATTACCCGAAGGGATTCCCTTGA(141) |
This study |
| PG0209 |
GGTGAGCACCACAAGAATCAAG/GCCGGAGCATACATTGCTATC(144) |
This study |
| PG1321 |
GAGGTGGCTATGCACAGGTAC/GTCGCAAGTATTGCGGTTCTG(147) |
This study |
|
groES
|
CCTCTCAAGGGTGAAGTAATCGCT/ATTTGCCGTAGAGTACGGTGTCTC(91) |
[42]
|
|
groEL
|
CGGCTACATCTCTCCCTACTTCGT/GAGGATCGGGAGCATCTCTTTCAG(121) |
[42]
|
|
clpB
|
CTGCTCAAGGCCGTTATGGATCA/CTGGTTGGTCTCATGCGACAGA(160) |
[42]
|
|
dnaK
|
CTGACCGGTGAGGTAAAGGATGTC/CTTCGTCGGGATAGTGGTATTGGC(120) |
[42]
|
|
htpG
|
CGGTCGCTGACCGTGATCGT/TCTCCAATGCCGTGGATGCT(132) |
[42]
|
|
EMSA probes
|
|
|
groES
|
CACCTTTTTTCCGACCGACT/TGTTGCTTGGTTTGTTATTGTTAGT(215) |
This study |
|
dnak
|
TGTACAGAGTCATTATTCGT/TCCCATAATTCTATATTCGT(199) |
This study |
|
htpG
|
AATAAAAATTTTCTCAGACCACGT/CATAGTGTATTATTATCTGTTAATGT(203) |
This study |
|
clpB
|
GTTCTGAGTTTCATAGTAGGGTA/GCTGTGTTTGTTGTTACCAGA(229) |
This study |
|
sod
|
GTTCGTGAGTCATAACGTCT/TTCGTCATGGAATGAATCGGT(164) |
This study |
|
ahpC
|
ACTGCGCCTTCTTAATGTTT/AGTATGAACGTTTGTTTCAGG(356) |
[9]
|
|
Strains and plasmids
|
|
|
Name
|
Description or Genotype
|
Reference
|
|
P. gingivalis
|
ATCC33277(type strain) |
Coykendall et al |
|
oxyR mutant |
ATCC33277ΔoxyR::ermF-ermAM
|
This study |
|
rprY mutant |
ATCC33277 rprY::ermF-ermAM
|
[9]
|
|
rprY tet deletion |
ATCC33277 ΔrprY::tetQ
|
This study |
|
E. coli DH5a |
fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
|
Invitrogen |
|
E. coli TB28 |
rph-1 ilvG rfb-50 lacZYA <frt >
|
[43]
|
| pRS414 |
lacZ protein fusion vector, lacZYA lacks promoter, RBS and translational start, apr, colE1 origin |
[44]
|
| pFD972a |
Expression vector, 9.1 kb; tetQ; (Apr) Tcr |
[45]
|
| pRprylacZ |
pFD972a rprY-lacZ
|
This study |
| pFD288 |
Bacteriodetes shuttle vector |
[15]
|
| pFDrprY |
pFD288rprY
|
This study |
