Table 2. Genes involved in the oxidative stress response that are differentially expressed under Na+ depleted conditions.
Parent ATCC 33277−/+Na+ | rprY mutant −/+Na+ | ||||||
Locusa | Common name | Fold expb | P valc | Repeats | Fold exp | P val | Repeats |
PG0034 | Thioredoxin | 2.95 | 0.0004 | 12 | 3.25 | 0.0001 | 12 |
PG0046 | Phosphatidate cytidylyltransferase | 2.21 | 0.0028 | 12 | 2.25 | 0.0001 | 12 |
PG0047 | Cell division protein FtsH, putative | 2.04 | 0.0026 | 12 | 2.12 | 0.0016 | 12 |
PG0095 | DNA mismatch repair protein MutS | 0.65 | 0.0806 | 8 | 1.91 | 0.0041 | 8 |
PG0209 | Formate-nitrite transporter | 3.71 | 0.0004 | 12 | 3.39 | 0.0241 | 11 |
PG0258 | ABC transporter, ATP-binding protein | 1.78 | 0.0028 | 12 | 3.94 | 0.0000 | 12 |
PG0275 | Thioredoxin family protein | 1.71 | 0.0049 | 12 | 4.09 | 0.0000 | 12 |
PG0432 | NOL1-NOP2-sun family protein | 2.82 | 0.0517 | 12 | 3.58 | 0.0001 | 12 |
PG0433 | Tetrapyrrole methylase family protein | 2.88 | 0.0165 | 12 | 7.43 | 0.0001 | 8 |
PG0618 | Alkyl hydroperoxide reductase, C subunit | 1.17 | 0.1644 | 12 | 1.85 | 0.0119 | 12 |
PG0889 | Peptidase, M24 family | 2.35 | 0.0004 | 12 | 2.94 | 0.0002 | 12 |
PG0890 | Alkaline phosphatase, putative | 2.37 | 0.0002 | 12 | 3.46 | 0.0000 | 12 |
PG1043 | Ferrous iron transport protein B | 0.96 | 0.7002 | 7 | 2.01 | 0.0501 | 9 |
PG1044 | Iron dependent repressor, putative | 1.50 | 0.2211 | 12 | 3.27 | 0.0067 | 8 |
PG1088 | Acetyltransferase, GNAT family | 2.14 | 0.0001 | 12 | 0.94 | 0.5204 | 12 |
PG1089 | DNA-binding response regulator RprY | 1.58 | 0.0001 | 12 | 2.05d | 0.0000 | 12 |
PG1124 | ATP:cob(I)alamin adenosyltransferase, putative | 1.12 | 0.4240 | 12 | 2.02 | 0.0000 | 12 |
PG1134 | Thioredoxin reductase | 1.27 | 0.0248 | 12 | 1.93 | 0.0075 | 12 |
PG1190 | Glycerate dehydrogenase | 1.56 | 0.1968 | 12 | 4.20 | 0.0000 | 10 |
PG1286 | Ferritin | 2.03 | 0.0005 | 12 | 4.00 | 0.0000 | 12 |
PG1321 | Formate–tetrahydrofolate ligase | 4.79 | 0.0001 | 12 | 6.78 | 0.0000 | 12 |
PG1545 | Superoxide dismutase, Fe-Mn | 1.96 | 0.0069 | 12 | 4.44 | 0.0001 | 12 |
PG1640 | DNA-damage-inducible protein F | 1.60 | 0.0011 | 12 | 2.16 | 0.0001 | 10 |
PG1642 | Cation-transporting ATPase, authentic frameshift | 1.88 | 0.0000 | 12 | 3.50 | 0.0000 | 12 |
PG1729 | Thiol peroxidase | 3.03 | 0.0000 | 12 | 3.68 | 0.0000 | 12 |
PG1841 | Conserved hypothetical protein | 3.20 | 0.0006 | 12 | 4.02 | 0.0005 | 12 |
PG1868 | Membrane protein, putative | 1.50 | 0.4660 | 10 | 3.83 | 0.0013 | 12 |
PG2008 | TonB-dependent receptor, putative | 1.03 | 0.8227 | 5 | 3.11 | 0.0620 | 8 |
PG2213 | Nitrite reductase-related protein | 1.54 | 0.0004 | 12 | 2.26 | 0.0005 | 12 |
Loci numbers are those from the annotation of the genome of strain W83 and the microarrays.
Fold expression.
P values were calculated using LIMMA.
The microarray PCR amplicon for rprY is upstream from the erythromycin resistance cassette that was used to generate the rprY mutation, thus an expression signal was still detectable.