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Measurements of cell viability using MTT reduction (mitochondrial viability) and LDH release into the medium and levels of glutamate uptake in primary rat astrocytes. Results are expressed as mean ± SEM % control. Note that although low concentrations of HHE were not toxic as measured by LDH release into culture medium or MTT reduction concentrations beginning at 2.5 μM led to a significant decrease in glutamate uptake.
