Fig. 2.

Extracellular UTP increases N-cadherin expression in a schwannoma cell line. a Extracellular UTP increased N-cadherin expression in a dose- and time-dependent manner. Schwann cell protein extracts (30 μg) treated for different time periods manner with UTP (250 μM) or with increasing concentrations of UTP for 24 h were resolved by SDS-PAGE, blotted, and probed with an N-cadherin antibody. Fold stimulation in N-cadherin expression is expressed as the mean ± SD (six independent experiments). Statistical significance: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. b Schwann cells treated with UTP (250 μM) for 12 h were mounted on coverslips and subjected to immunocytochemistry against N-cadherin (green). Up-regulation of N-cadherin in cell–cell contacts (dotted lines) was visualised by fluorescence microscopy. Nuclei were stained with Hoechst 33342 (blue). Scale bars: 20 μm