Table II.
Measurement | Units | II | IAT | positive controls (mean) |
---|---|---|---|---|
Metabolic stability | ||||
half-life in human liver microsomes | min | >60 | 28 | Verapamil (12) |
Permeability | ||||
Papp(A→B) in CaCO-2 assay | 10−6 cm/s | 36 | 99 | Propranolol (19) |
Aqueous solubility | μg/ml | >75 | 19 | SMART-H (1.1) |
Drug-drug interactions | ||||
IC50 value for Cyp3A4 (substrate: Testosterone) | μM | 20 | 5.5 | Ketoconazole (0.02) |
IC50 value for Cyp2D6 (substrate: Dextromethorhan) | μM | >50 | 34 | Quinindine (0.1) |
IC50 value for Cyp2C19 (substrate: (S)-mephennytion) | μM | 6.6 | 5.3 | Ticlopidine (0.37) |
IC50 value for Cyp2C9 (substrate: Diclofenac) | μM | 17 | 4.9 | Sulfaphenazole (0.5) |
IC50 value for Cyp1CA2 (substrate: Phenacetin) | μM | 9.2 | 8.1 | Furafylline (2.2) |