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. Author manuscript; available in PMC: 2013 Dec 14.
Published in final edited form as: J Immunol Methods. 2012 Aug 28;386(0):10–21. doi: 10.1016/j.jim.2012.08.011

Fig. 5.

Fig. 5

Comparison of results with different CD3 gate placements. Plots are from a representative replicate from each day for one animal. A) The top row depicts the gate used for the qualification analysis. The bottom row shows how the gates were adjusted to include the CD3 low cells. B) The CD3 low cells express CD4. C) Frequencies of CD4 and CD8 T cells by both gating methods. D) Frequencies of cytokine+ CD4 and CD8 T cells by animal.