Table 4.
Pre-qualification precision results. Coefficients of variation (CVs) were computed from linear mixed effects models using the natural log of the response. One model was fitted per animal (3 animals) for each cell type/stimulation/cytokine combination. Values are shown for animals with an overall mean≥0.1%. N/A means no animals with mean≥0.1% for that measurement.
| Measurement | Mean response (% of CD4 or CD8 T cells) |
Avg CV% day |
Avg CV% vial |
Avg CV% operator |
Avg CV% residual error |
Avg CV% total |
|---|---|---|---|---|---|---|
| CD4 IFNγ | 0.17 | 7.8 | 0 | 29.6 | 19.2 | 38.3 |
| CD4 IL-2 | 0.20 | 5.5 | 0 | 26.1 | 16.6 | 32.3 |
| CD4 TNF | 0.22 | 7.2 | 0 | 32.3 | 19.2 | 40.0 |
| CD8 IFNγ | 0.10 | 16.5 | 8.8 | 14.8 | 18.9 | 31.0 |
| CD8 IL-2 | N/A | N/A | N/A | N/A | N/A | N/A |
| CD8 TNF | 0.15 | 15.8 | 0 | 10.8 | 17.9 | 26.5 |