Fig. 2.

Expression of regulatory T cell (T_reg)-associated markers. (a) Expression of forkhead box protein 3 (FoxP3) on CD4⁺ resting cells from a representative glutamic acid decarboxylase (GAD)-alum-treated subject; (b) expression of FoxP3 in GAD-stimulated cells, gates indicated above plots. (c) Expression of FoxP3 on CD4⁺ cells (placebo n = 25, GAD-alum n = 24); (d) the fraction of CD4⁺ cells co-expressing CD25^hiCD127^loFoxP3⁺CD39⁺ from placebo (n = 24) and GAD-alum (n = 22) patients. Each point represents an individual and median values are indicated with horizontal lines. Statistical significances are shown as P-values.