Fig. 3.

Dose- and time-dependent effects of tumour necrosis factor (TNF)-α on interleukin (IL)-37b mRNA expression in T84 cells. (a) Cells were stimulated for 24 h with increasing concentrations of TNF-α, and then the IL-37b mRNA expression was analysed by real-time polymerase chain reaction (PCR) (b) Cells were stimulated with TNF-α (100 ng/ml) for different time-periods, and then the IL-37b mRNA expression was analysed by real-time PCR. All values are expressed as means ± standard deviation (n = 3).**P < 0·01 and *P < 0·05; a significant difference from the values for medium alone.