. 2013 Apr 18;172(3):444–454. doi: 10.1111/cei.12067

Table 1.

Representative fluorescence activated cell sorter (FACS) mature phenotype markers for dendritic cells (DCs).

	Control		LPS								Hypoxia

	Control		LPS		LPS+PSC		LPS+MK		LPS+PBN		H		H + PSC		H + MK		H + PBN

	%	MFI	%	MFI	%	MFI	%	MFI	%	MFI	%	MFI	%	MFI	%	MFI	%	MFI
Presentation molecules
MHC II	96·5	549·9	98·6	2260·9^a	96·9	1106·6^b	94·2	700·7^b	91·8	484·2^b	99·2^b	1085·2	93·1	660·7	75·9	160·9	82	367·9
Co-stimulatory/signalling molecules
CD40	64·8	35·8	90·9^a	95·1^a	80·2	47·2^b	73·8^b	43·1^b	59·8^b	36·2^b	91·3^a	78·2^a	67·9^c	36·6^c	48·5^c	25·4^c	40·3^c	23·5^c
CD80	5·6	10·5	69·2^a	48·8^a	24·2	19^b	36·3^b	25·4^b	23·7^b	19·4^b	28^a·^b	22·1^a·^b	3	8·7^c	3·2	10·1	35·3	10·5
CD86	90·7	317·7	97·5	855·6^a	97	592·8	90·5	326·2^b	86·7	404·3^b	96·3	389·3^b	88·4	391·1	71·5	143·5	81·5	321·7
CD54	98·8	780·2	99·3	5343^a	98·5	2629·5^b	98·1	1416·6^b	97·7	1197^b	96·8	2504·4^a·^b	96·2	867·4^c	97·7	673^c	99·3	1197^c
Maturation antigen
CD83	12·4	16·4	49·7^a	47·3^a	17·4^b	18·8^b	16·6^b	19·1^b	34·9^b	26·5^b	54·1^a	43·8^a	5·2^c	12·1^c	5·4^c	13·3^c	18^c	19·1^c

Versus C P < 0·05,

versus lipopolysaccharide (LPS) P < 0·05,

versus H P < 0·05. Hypoxia and LPS conditions cause phenotypic and morphological changes in immature dendritic cells (iDCs). Stimulated DCs were analysed by flow cytometry for CD40, CD80, CD83, CD86, CD54 and human leucocyte antigen D-related (HLA-DR) surface marker expression. Mean fluorescence intensity (MFI) for hypoxia-DC and LPS-DC was higher than for non-stimulated cells, although conventional markers showed lower MFI intensity under hypoxia compared to LPS. MFI and expression of each marker are presented as mean ± standard deviation of six experiments. MHC: major histocompatibility complex; PSC: P-glycoprotein inhibition using valspodar; PBN: probenecid; MK: CAS 115104-28-4.