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Simultaneous blockade of programmed death (PD)-1 and vascular endothelial growth factor receptor 2 (VEGFR2) induced synergistic anti-tumour effect in vivo. BALB/c mice were inoculated subcutaneously with Colon-26 cells and were given with control rat immunoglobulin (Ig)G, anti-PD-1 monoclonal antibody (mAb), anti-VEGFR2 mAb or both mAbs five times (arrow). Data are presented as mean ±standard error of seven to 10 mice of each group. *P < 0·05; **P < 0·01.
