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. 2013 Jun;54(6):1531–1540. doi: 10.1194/jlr.M031591

Fig. 4.

Fig. 4.

Depletion of JNK2, but not JNK1, attenuates insulin-induced upregulation of SREBP-1c target genes and increase of fatty acid synthesis. A: Effects of JNK1 or JNK2 depletion on SREBP-1c target gene expression. Differentiated adipocytes were treated with control siRNA (siControl), JNK1 siRNA (siJNK1), or JNK2 siRNA (siJNK2) in maintenance medium for 7 days. Cells were then starved in serum/Dex/insulin-free maintenance medium for 16 h followed by stimulation with or without 100 nM insulin for 24 h. The mRNA expression levels of each gene were measured by quantitative real-time PCR. Data are presented as means ± SEM of three independent experiments. **P < 0.01 versus siControl without insulin; ##P < 0.01 versus siControl with insulin. B: Analysis of fatty acid synthesis. Data are presented as means ± SEM of three independent experiments. **P < 0.01 versus siControl without insulin; ##P < 0.01 versus siControl with insulin.