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. 2013 Jun;54(6):1531–1540. doi: 10.1194/jlr.M031591

Fig. 5.

Fig. 5.

Depletion of SREBP-1 attenuates insulin-induced upregulation of lipogenic enzymes and fatty acid synthesis. A, B: Effects of SREBP-1c depletion on expression of lipogenic enzymes. Differentiated adipocytes were treated with control siRNA (siControl), or SREBP-1 siRNA (siSREBP-1) in maintenance medium for 7 days. Cells were then starved in serum/Dex/insulin-free maintenance medium for 16 h followed by stimulation with or without 100 nM insulin for 24 h. The mRNA expression levels of lipogenic enzymes (A) and SREBP-1c (B) were measured by quantitative real-time PCR. Data are presented as means ± SEM of three independent experiments. **P < 0.01 versus siControl without insulin; #P < 0.05, versus siControl with insulin. C: Analysis of fatty acid synthesis. Data are presented as means ± SEM of three independent experiments. **P < 0.01 versus siControl without insulin; ##P < 0.01 versus siControl with insulin.