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Nitrosylation is required for normal interaction of ARH with AP-2. Cells were treated or not with 4 mM L-NAME for 30 min, and then lysed and immunoprecipitated with antibodies to ARH or AP-2. Immunoprecipitants were run on SDS-PAGE and immunoblotted for the presence of ARH, AP-2, and clathrin heavy chain (CHC).
