Figure 6.

TEM and Immunogold EM of VSV-Infected A549-IFITM3 and A549-IFITM3-VAPA Cells

(A and B) A549-IFITM3 and A549-IFITM3-VAPA were mock infected (left panel) or with high titers of VSV for 1 hr (rest of panels). Then cells were washed to remove unbound viruses and subjected to TEM (scale bar, 0.1 μm) and immunogold EM (scale bar, 0.2 μm) as described in the Experimental Procedures. Dual immunogold labeling was performed with anti-Myc antibody and 15 nm gold particle-conjugated secondary antibody (IFITM3, filled triangle) and anti-VSVgp antibody and 30 nm gold particle-conjugated secondary antibody (VSVgp, open arrow). The areas of particular MVBs are marked by dashed lines. See also Figure S6.