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. 2013 May 6;210(5):1003–1019. doi: 10.1084/jem.20120521

Figure 8.

Figure 8.

Effect of coexpression of JAK2V617F and mutant NF-E2 in vivo. (A) Experimental design. Murine bone marrow was retrovirally transduced with a vector expressing JAK2V617F and transplanted into lethally irradiated balb/c animals. 10 wk after transplantation, bone marrow was harvested and infected with an empty control lentivirus, pLeGO-iC2, or with a lentivirus expressing the 262aa NF-E2 truncation mutant (pLeGO-iC2-NF-E2-262aa). Cells expressing both JAK2V617F and the 262aa NF-E2 mutant or empty control virus were FACS sorted. Subsequently, 50,000 double-positive cells were transplanted together with 300,000 bone marrow support cells into lethally irradiated recipient mice. (B–E) Peripheral blood was obtained by retro-orbital puncture and analyzed on an Advia 120 system. (n = 3–7 mice per genotype). Mean and SEM are shown. (B) Hemoglobin. (C) Mean corpuscular volume (MCV). (D) Mean corpuscular hemoglobin (MCH). (E) WBC count. Statistical analysis was conducted using Student’s t tests. *, P < 0.05; ***, P < 0.001. (F–I) H&E staining of formalin-fixed and paraffin-embedded organ sections. (F and G) Mice expressing JAK2V617F alone (with the empty pLeGo-iC2 control). (H and I) Mice expressing both JAK2V617F and the NF-E2-262aa mutant. Bars: (F and H) 100 µm; (G and I) 50 µm.