Figure 4.

(A) Effect of intact sorafenib or sorafenib released from the sorafenib-loaded film on the MMP-2/9 activity of HuCC-T1 cells. The cells were exposed to various concentrations of sorafenib for 32 hours, and then the conditioned media were used for gelatin zymography. (B) Effect of intact sorafenib (a and b), media taken from unloaded PCL film (c) and sorafenib released from the sorafenib-loaded film (d) on the invasion of HuCC-T1 cells. All groups H&E stained, 100×. Invasiveness of HuCC-T1 cells was assessed with Matrigel invasion assay. (b) The number of cells in four randomly selected microscopic fields per membrane was counted. 2 × 10⁴ HuCC-T1 cells in the RPMI 1640 media (without FBS) were seeded onto the upper chamber, and the lower chamber was filled with RPMI 1640 media (with FBS 10%).