Table 2.
Effect of O-antigen on cellular deformities
Strains* | PBPs deleted |
O8-antigen | Deformities (% population) |
Total cells |
---|---|---|---|---|
CS315-1 | 4 7 5 | − | 50 | 529 |
AG375-3 | 4 7 5 | + | 23 | 707 |
AG375-3* | 4 7 5 | − | 29 | 750 |
CS446-1 | 4 7 5 6 | − | 49 | 408 |
AG456-1 | 4 7 5 6 | + | 36 | 414 |
AG456-1* | 4 7 5 6 | − | 49 | 432 |
Strains designated with the ‘CS’ prefix were derived from Escherichia coli K-12, which lacks the O-antigen (Liu & Reeves, 1994). Strains designated with the prefix ‘AG’ was derived from E. coli 2443, which expresses the O8-antigen (Rick et al., 1994). AG names followed by an asterisk (*) designate strains from which the O8-antigen genetic locus was deleted and replaced by mutated genes from E. coli K12.