Table 4.

Complementation of shape defects by heterologous O-antigen

Strain	Plasmid	O-antigen	Cells (Total #)	Normal (%)	Abnormal (%)	Branched (%)
CS446-1	None	−	1008	29	60	11
	pBluescript	−	594	9	82	9
	pWQ5	Klebsiella O1	952	22	76	2
CS703-1	None	−	1313	23	73	4
	pBluescript	−	498	9	87	4
	pWQ5	Klebsiella O1	359	28	69	3

CS446-1 and CS703-1 are Escherichia coli K12 strains lacking four and seven pencillin-binding proteins, respectively.

The O-antigen was introduced into these strains on plasmid pWQ5 (Vinés et al., 2005), and cells categorized as in Fig. 1.