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. Author manuscript; available in PMC: 2014 Apr 23.
Published in final edited form as: Biochemistry. 2013 Apr 10;52(16):2774–2782. doi: 10.1021/bi400150x

Figure 3.

Figure 3

ZF TEDOR spectra show that the pore binding site is correlated with chemical shift changes. A 400 MHz DNP enhanced TEDOR spectrum (a) with 12.5 ms mixing is shown in blue. The wild type M2 sequence was used, and ~30% of the sample was drug bound as indicated by chemical shifts. In b), a similar spectrum is shown of D21G, D24G M2 but with the protein kinetically trapped in the apo state. Notably, the pore site at G34 is not detected in the functionally unbound sample shown in blue in (b). In red, the TEDOR spectrum acquired at ~278 K, 500 MHz, and 8.8 ms mixing of D21G, D24G M2 is presented for comparison. All possible assignments are listed for each peak in blue (DNP) and red (non-DNP).