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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Mol Microbiol. 2012 Jul 26;85(6):1119–1132. doi: 10.1111/j.1365-2958.2012.08163.x

Figure 4. Redistribution of ExPortal proteins following sub-lethal polymyxin B challenge.

Figure 4

The distribution of SecA (A, B, C) and HtrA (D, E, F) on S. pyogenes SF370 was assessed by immunogold electron microscopy following challenge with polymyxin B at the concentrations indicated in the Figure (scale bar = 200nm). The distribution of HtrA was also assessed by immunofluorescent microscopy in the absence of (G, H) and following challenge with polymyxin B at 47 μM (I, J) and was quantitated as described previously (scale bar = 500nm) (K). Data represents the mean and SEM derived from at least 3 independent experiments and examination of a minimum of 1000 stained cells. In untreated cultures, the number of SF370 or HSC5 cells with a single focus was significantly higher than any other staining pattern (P < 0.05), whereas in polymyxin B-treated cultures, the number of streptococcal cells with multiple foci was significantly higher (P < 0.05).