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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Mol Microbiol. 2012 Jul 26;85(6):1119–1132. doi: 10.1111/j.1365-2958.2012.08163.x

Figure 7. Challenge with HNP-1 results in re-distribution of HtrA.

Figure 7

Cultures of S. pyogenes HSC5 were challenged with the indicated concentrations of HNP-1 and subjected to immunofluorescent microscopy to assess the distribution of HtrA (A, B). Cell walls were visualized by staining with fluorescent vancomycin (scale bar = 1μm). Staining patterns were quantitated as described previously (C). Data represents the mean and SEM derived from at least 3 independent experiments and examination of a minimum of 1000 stained cells. Challenge with the peptide significantly decreased the number of SF370 or HSC5 cells exhibiting HtrA at single foci (P<0.0001) and resulted in a significant increase in the number of multiple foci (P<0.0001) in the SF370 strain.