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. 2012 Sep 19;32(38):13125–13136. doi: 10.1523/JNEUROSCI.1937-12.2012

Figure 2.

Figure 2.

Amyloid plaques are increased by Abca1 hemizygosity in APP/E4 mice but not in APP/E3 mice. Amyloid plaques were compared in APP/E3 and APP/E4 as well as in APP/E3/Abca1−/+ and APP/E4/Abca1−/+ mice between 6 and 8 months of age (mean age, 7.5). A, Brain sections were stained with X-34 to visualize compact fibrillar amyloid plaques. Representative pictures for X-34 staining are shown above the graphs (20× magnification). X-34-positive amyloid load analyzed by two-way ANOVA and Bonferroni's posttest. There is an interaction between Abca1 and ApoE genotypes (p < 0.05) and a significant effect of ApoE genotype (p < 0.001), but no effect of Abca1 genotype. Note that the level of compact amyloid plaques in APP/E4/Abca1−/+ is increased by ninefold compared with the level in APP/E3/Abca1−/+ mice (Bonferroni's posttest, p < 0.05). *p < 0.05 and **p < 0.01, two-group comparisons by t test. N = 8–12 mice per group. B, Brain sections were stained with anti-Aβ antibody, 6E10, to visualize diffuse and compact amyloid plaques. Representative pictures for 6E10 staining are shown above the graph (20× magnification). Analysis is by two-way ANOVA and Bonferroni's posttest. There is no interaction between Abca1 and ApoE genotypes, and there is a significant effect of ApoE (p < 0.01), and Abca1 (p < 0.05) genotypes; p < 0.05, by Bonferroni's posttest. Note that APP/E4 have more than twofold increase of plaque load (*p < 0.05 by t test), and APP/E4/Abca1−/+ mice have more than threefold increase in amyloid load versus APP/E3/Abca1−/+ (*p < 0.05 by t test). N = 8–13 mice per group. Error bars indicate SEM.