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. 2012 Sep 19;32(38):13125–13136. doi: 10.1523/JNEUROSCI.1937-12.2012

Figure 4.

Figure 4.

ApoE and ApoA-I levels in soluble brain fraction are decreased in APP/E4/Abca1−/+ but not in APP/E3/Abca1−/+ mice. Soluble brain exacts were used from the cortices and hippocampi of 6- to 8-month-old APP/E3, APP/E4, APP/E3/Abca1−/+, and APP/E4/Abca1−/+ mice. A, Cholesterol concentration was measured in the soluble brain fraction as explained in the text. Two-way ANOVA demonstrated no interaction and no effects of genotypes. N = 6–9 mice per group. B, ApoE was detected in the soluble fraction of cortex and hippocampus by ELISA as explained in Materials and Methods. Analysis is by two-way ANOVA. There is no interaction between ApoE and Abca1; there are main effects of Abca1 (p < 0.01) and ApoE (p < 0.001). *p < 0.5 by t test. N = 6–10 mice per group. C, ApoA-I was detected in the soluble fraction of cortex and hippocampus by WB. The intensity of the bands were quantified and analyzed by two-way ANOVA. There is no interaction between ApoE and Abca1, and there is a main effect of Abca1 (p < 0.05) but not of ApoE. *p < 0.5 by t test. N = 6–8 mice per group. D, Western blotting for Abca1, full-length APP (APPfl), and C-terminal fragments result of β-secretase cleavage (CTFβ). β-Actin is shown as a loading control. E represents the quantification of CTFβ. Note that there is no difference between the genotypes. N = 6–8 mice per group. Error bars indicate SEM.