Figure 3.

Phylogenetic tree of 16S rRNA gene sequences, representing the bacterial species that were isolated from chironomid egg masses and larvae. These species demonstrated the ability to grow in high metal concentrations. All the isolate names reveal their origin as follows: EM - egg mass, L - larva, Cu - copper resistance, Cr - Chromate resistance, Zn - zinc resistance, Pb - lead resistance. The sequences alignments were performed using CLUSTAL W program and the trees were generated using the neighbor-joining method with Kimura 2 parameter distances in MEGA 4.1 software. Bootstrap values (from 1,000 replicates) greater than 50% are shown at the branch points. The bar indicates 5% sequence divergence. High quality figures are available online.