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. Author manuscript; available in PMC: 2013 May 7.
Published in final edited form as: Histochem Cell Biol. 2012 Apr 17;138(1):113–125. doi: 10.1007/s00418-012-0952-1

Fig. 6.

Fig. 6

Localization of GRP-78 in the extracellular matrix. a, e Expression of GRP-78 and DMP1 in the biomimetic ECM secreted by differentiating HMSCs (a) and DPSCs (e). b, f Expression of fibronectin in the biomimetic ECM secreted by differentiating HMSCs (b) and DPSCs (f). c, g Secondary antibody negative controls. d, h Images showing lack of DAPI fluorescence in the sections showing absence of cellular DNA. i Representative fluorescence micrograph showing colocalization of secreted GRP-78 (green) on collagen fibrils (red) in the biomimetic ECM of differentiating HMSCs. j GRP-78 immuno-blots of the secretome from HMSCs and T44 odontoblasts after 0, 7, 14, and 21 days in differentiation media. k Densitometric quantification of the blots in figure (j) showing fold change in GRP-78 secretion. The empty bars represent GRP-78 secreted by HMSCs and the filled bars represent GRP-78 secreted by T4-4 odontoblasts