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Imaging cell surface protein in live HeLa cells with ODF-HaloTag ligands (A-C) by confocal microscopy (excitation 405 nm; bar denotes ca. 20 microns). (D,E) SDS PAGE gel of cell extracts showing labeling of 66.8 kD cell surface protein (D: fluorescence scan; E: Coomassie blue staining). Lane 1: size marker, lane 2: control HeLa cells, lane 3: HeLa cells expressing cell surface protein.
