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. 2013 Feb 7;6:20. doi: 10.1186/1754-6834-6-20

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Copyright © 2013 Roach et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Turbidity reduction analysis of LysA, LysA2, LysgaY and λSa2 endolysin against multiple lactobacilli in a range of pH and ethanol concentrations. a) Effect of pH on turbidity reduction specific activities (OD_600nm/min/μM; as described by [49]). b) Effect of ethanol on lysin activity (normalized to specific activity achieved at pH 5.5 in panel a). Live cells of L. fermentum isolate 0605-B44 (blue), L. fermentum isolate BR0315-1 (green), and L. brevis isolate 0605–48 (red) were used as substrate. Data represent the average of three experiments (n=3) ± SEM.