Skip to main content
. 2013 Feb 7;6:20. doi: 10.1186/1754-6834-6-20

Table 1.

Activity of LysA, LysA2, LysgaY and λSa2 endolysin against lactic acid bacteria as determined by turbidity (OD600nm) reduction analysis

 
 Activitya
  
Strain LysA LysA2 LysgaY λSa2 Strain Source
Gram positive
  
  
  
  
  
Lactobacillus amylovorus B-4540
 -
 ++
 ++
 ++
 NRRLb
Lactobacillus brevis 0605-48
 ++
 ++
 ++
 ++
 this studyc
Lactobacillus delbrueckii subsp delbrueckii B-763
 -
 -
 +
 +
 NRRL
Lactobacillus fermentum BRO315-1
 ++
 +
 +
 ++
 this study
Lactobacillus fermentum BRO315-25
 ++
 +
 +
 +++
 this study
Lactobacillus fermentum 0605-B44
 +++
 +
 +
 +++
 this study
Lactobacillus fermentum 0713-3
 +++
 +
 +
 +++
 this study
Lactobacillus gasseri B-4240
 +++
 +
 +
 +++
 NRRL
Lactobacillus hilgardii B-1843
 -
 -
 -
 -
 NRRL
Lactobacillus malefermentans B-1861
 -
 -
 -
 ++
 NRRL
Lactobacillus paracasei BRO315-44
 -
 -
 -
 -
 this study
Lactobacillus reuteri B-14171
 ++
 +
 ++
 +
 NRRL
Staphylococcus aureus Newman
 +
 -
 +
 +++
 Jean C. Leed
Staphylococcus epidermidis
 +
 -
 ++
 +++
 USDA
Staphylococcus hyicus
 -
 -
 -
 +
 USDA
Staphylococcus warneri
 +
 -
 +
 +++
 USDA
Staphylococcus xylocus
 ++
 -
 ++
 +++
 USDA
Streptococcus agalactiae
 ++
 -
 ++
 +++
 David Pritcharde
Streptococcus dysgalactiae
 ++
 -
 ++
 +++
 USDA
Streptococcus pyogenes
 +
 -
 +
 +++
 Dan Nelsonf
Streptococcus suis 531-668
 +++
 -
 +++
 +++
 Dan Nelsonf
Weisella viridescens B-1951
 -
 -
 +
 +++
 NRRL
Yeast
  
  
  
  
  
Saccharomyces cerevisiae Y-2034
 -
 -
 -
 -
 NRRL
Gram negative
  
  
  
  
  
Escherichia coli DH5α - - - - Invitrogen

a As measured by OD600nm, decrease of whole cell suspensions (initial OD600nm = ~1) treated with lysin (10μM) for 30 min. “+++” between 100% and 75% decrease, “++” between 74% and 50% decrease, “+” less than 49% decrease, “−“no decrease, with respect to no lysin control.

b ARS Culture Collection, Peoria, IL (also known as the NRRL Collection).

c Isolated from fermentors at a commercial dry-grind ethanol facility as described previously [6]. L. fermentum BR0315-1, BR0315-25, and 0713–3 were planktonic isolates, and their MICs for virginiamycin are 16 μg/ml, ≤2 μg/ml, and ≤2 μg/ml, respectively. L. fermentum 0605-B44 was a biofilm isolate from coupon scrapings of a Centers for Disease Control biofilm reactor inoculated with a mash sample from the fermentor.

d Channing Lab, Womens and Brigham Hospital, Boston, MA.

e Dept. Biochemistry, Medical School, Univ. Alabama, Birmingham.

f Department of Veterinary Medicine, UMD, College Park MD.