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. 2013 May 7;8(5):e63442. doi: 10.1371/journal.pone.0063442

Figure 5. Isothermal GdmCl denaturation.

Figure 5

Isothermal GdmCl denaturation studies were carried out at 25°C for WT Trx (○), pelB Trx (•) and malE Trx (▾) in CGH-10 buffer (pH 7.4) (A) Fluorescence change as a function of GdmCl concentration. The continuous line through the data is a fit of the data to a two-state unfolding model. (B) Fraction unfolded (fu) as a function of denaturant concentration. The continuous line through the data is a fit of the data to a two-state unfolding model, using a m-value of 3.40 kcal mol−1 M−1. Values of ΔG° were 8.9±0.1 and 8.7±0.1 kcal mol−17.5±0.1 kcal mol−1for WT Trx, pelB Trx and malE Trx respectively. Corresponding Cm values were 2.60, 2.5 M and 2.2M respectively.