Figure 6. Localization of CB₁ and CB₂ receptors in mesenteric arteries.

Panels show confocal microscopic images of von Willebrand factor (vWF, red), DAPI (blue), CB₁ and CB₂ receptors (green) in mesenteric arteries from lean Zucker rats (LZRs, A and B) and obese Zucker rats (OZRs, C and D). Endothelium-intact mesenteric sections were immunolabeled with antibodies against CB₁, CB₂ and von Willebrand factor. The nuclei were counterstained with DAPI. An overlay of the vWF, DAPI and CB₁ or CB₂ images is presented (merge). Endothelial localization of CB₁ and CB₂ receptors is shown in the merged images (yellow). The images are representative of three separated experiments. N = 5/group. Scale bar = 40 µm.