Figure 5. ChIP analysis using streptavidin beads of the hKlf4 expressing mES cells.

The cells were cultured with or without LIF in the absence of Dox for 1.5 days. The Nanog upstream 4.7 kb enhancer (Nanog4.7) was enriched by the induction of biotinylated hKlf4. The Nanog upstream 3.3 kb region (Nanog3.3) was used as a control. Bars represent the average±SEM of at least two independent experiments with two repeats each. *, p<0.05.