Skip to main content
. 2013 May 7;8(5):e63758. doi: 10.1371/journal.pone.0063758

Figure 4. Decreased ATP sensitivity in both hetDel and hetT, del channels.

Figure 4

(A) Representative currents recorded by inside-out excised patch-clamp technique from COSm6 cells expressing WT channels and hetT, del mutants. Patches were exposed to different concentrations of Mg-free ATP as indicated. (B) ATP dose-response relationships, fit by Hill equation as described in methods. Data points indicate means ± SEM of n = 5–7 patches. * indicates P<0.05 compared with WT by One-Way ANOVA analysis. The fitted K1/2 for WT, homS225T, hetS225T, hetDel, and hetT, del channels are 13.75, 25.06, 14.8, 22.87 and 43.94 (in μM) and the Hill coefficients are 1.6, 1.3, 1.1, 1.28 and 1.2, respectively.