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. 2013 May 7;8(5):e61396. doi: 10.1371/journal.pone.0061396

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© 2013 Vasireddy et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Plots representative of 4 peripheral blood (PBMC)-derived iPSC clones. (A) iPSC reprogramming timeline (B) phase contrast image of established iPS clone with normal G-banded karyotype (C) Extracellular pluripotency markers; SSEA3/SSEA4, TRA-1-60; (D) Molecular profile of iPSC lines. Real-time PCR analysis shows expression of common pluripotency genes: DNMT3B, REX1, OCT4, NANOG. (E) Hematoxylin and eosin staining of CHM iPSC-derived teratomas displays 3 germ layers; endoderm, mesoderm, ectoderm. Arrows in panel E point towards two different germ layers in one image.