Figure 6. Eg5 is involved in the role of parkin in pancreatic cancer development. (A) Cells were transfected with the indicated siRNAs, and cell proliferation was examined by sulphorhodamine B staining. (B) Cells were transfected with the indicated siRNAs, and the percentage of BrdU-positive cells was examined. (C) Cells transfected with the indicated siRNAs were cultured in agarose for 2 wk, and the number of colonies formed was counted. (D) Immunohistochemical staining of parkin and Eg5 on serial sections of pancreatic adenocarcinomas. (E) Experiments were performed as in (D), and the expression of parkin and Eg5 was scored as low (+), medium (++) and high (+++) levels. Correlation coefficient (r) and p values were calculated by Spearman’s rank correlation test.