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. 2013 Mar 8;12(7):1142–1150. doi: 10.4161/cc.24216

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Copyright © 2013 Landes Bioscience

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graphic file with name cc-12-1142-g2.jpg — Figure 2. Effects of MBTD1 or Pr-Set7 depletion in mouse oocyte meiotic maturation and correlation of MBTD1 and Pr-Set7. (A) Microinjection of MBTD1 siRNA, Pr-Set7 siRNA or H4K20me1 antibody caused significant decrease in the GVBD rate of mouse oocytes (p < 0.05). (B) Western blotting results demonstrated the decreased expression level of Pr-Set7, 53BP1 and H4K20me1 after MBTD1 depletion. (C) Western blotting results demonstrated the decreased expression of 53BP1 after Pr-Set7 depletion. (D) Co-IP result showing the MBTD1 correlating with Pr-Set7 to function in mouse oocyte.