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. 2013 Mar 8;12(7):1142–1150. doi: 10.4161/cc.24216

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graphic file with name cc-12-1142-g5A_C.jpg — Figure 5A–C. Effects of MBTD1 or Pr-Set7 depletion on chromatin/chromosome configuration in mouse oocyte meiotic maturation. (A) PI staining micrographs showing abnormal chromatin after MBTD1 (b, c, d) or Pr-Set7 (b’, c’, d’) depletion; a and a’ are the controlled normal chromatin of MBTD1 and Pr-Set7, respectively.(B) Percent bars showing that 26.31% (MBTD1) and 25% (Pr-Set7) of the oocytes displayed abnormal chromosome configurations (UN, unidentified nucleolus) besides SN (surrounding nucleolus) and NSN (non-surrounding nucleolus) in mouse oocytes at the GV stage. (C) Confocal micrographs showing abnormal chromosome alignment in mouse oocytes at the MII stage after MBTD1 (b, c, d) or Pr-Set7 (b’, c’, d’) depletion; a and a’ are the controlled normal chromosomes alignment of MBTD1 and Pr-Set7, respectively. Spindle (green) and DNA (red). Arrows point out the abnormal aggregated chromosome. Bar = 10 μm.