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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Biochimie. 2013 Jan 16;95(6):1177–1184. doi: 10.1016/j.biochi.2013.01.005

Figure 1.

Figure 1

HGF stimulates the NADPH oxidase activity. (A) Primary mouse hepatocytes were treated with 50 ng/ml HGF for indicated times and NADPH oxidase activity was assayed as depicted under Materials and Methods. Each column represents the mean ± SE of at least four independent experiments carried out by triplicate. (B) HGF increases production of NADPH oxidase-derived peroxides. Hepatocytes were pretreated, or not with DPI 10 μM for 30 min before stimulation with 50 ng/ml HGF. De-esterified DCFH fluorescence was monitored at the indicated times after 30 min incubation with DCFH (5 μM). Each point represents the mean ± SEM of at least four independent experiments carried out by triplicate. * P ≤ 0.05 vs not treated cells (NT); # P ≤ 0.05 vs 30 min of HGF treatment.