Table 1. Molar concentrations, number of AMP molecules associated with each lipid, and number of AMP molecules in the inter-lamellar space between two lipids (assuming that approximately every second bilayer excludes AMP molecules).

AMP:DMPC	AMP/lipid	AMP/lipids inter-lamellar	Lipid/AMP Fraction	Area per lipid molecule (Å2)	# of layers calculated	# of layers observed
0∶1	0	0	–	40.8	–	–
1∶2	1	2	0.76∶1	40.0	1.4	1
1∶1	2	4	0.67∶1	40.0	2.8	1,2
2∶1	4	8	0.89∶1	40.0	5.9	2,4,8
3∶1	6	12	0.78∶1	40.0	8.5	8,9

The Lipid/AMP fraction refers to the ratio of lipid correlation peaks in Figure 3 A, normalized to the pure lipid peak. The positions of these lipid peaks were used to calculate the area per lipid molecule, which increased from 40.0 to 42.8 Å with AMP present. The area per AMP was calculated by multiplying the unit cell parameters of the tetragonal unit cell to 30.0 Å. The number of layers between a bilayer as obtained from calculation and from the data in Figure 4 is also included.