Table 1. Effect of 400, 600 and 800 nM of TSA on craniofacial development.
| 16 hpf- 3.5 dpf | ||||
| 800 nM | 600 nM | 400 nM | Control | |
| Mandibular | +/− | + | + | +++ |
| % | 58/95 = 61.05% | 100% | 100% | 100% |
| Hyoid | +/− | + | + | +++ |
| % | 32/95 = 33.6% | 100% | 100% | 100% |
| CB1 | – | + | + | +++ |
| % | 0% | 73.61% | 92.03% | 100% |
| CB2 | – | + | + | +++ |
| % | 0% | 52.77% | 84.06% | 100% |
| CB3 | – | +/− | +/− | +++ |
| % | 0% | 0% | 53.62% | 100% |
| CB4 | – | – | – | +++ |
| % | 0% | 0% | 4.35% | 100% |
| CB5 | – | – | – | +++ |
| % | 0% | 0% | 0% | 92.55% |
| Shape of elements | + | + | + | +++ |
| N | 95 | 144 | 138 | 161 |
Embryos were assigned the following groups based on the severity of craniofacial malformations when compared to wild-type embryos;+++ wild-type, +++/− Mild malformation,++ Moderate malformation,+Severe malformation, - Jaw elements absent or not stained.
Percentages reflect proportion of embryos with alcian blue stained cartilage element to the total number of treated embryos expressed as a percentage.