Table 2. Temporal requirements of HDAC function during craniofacial development.
| 24 h treatments with 800 nM TSA at different stages. Treated and control embryos were fixed at 3.5 dpf (84 hpf) | ||||
| 16–40 hpf | 28–52 hpf | 48–72 hpf | Control | |
| Mandibular | +++/− | + | +++/− | +++ |
| % | 98.8% | 86.3% | 99.3% | |
| Hyoid | +++/− | + | +++/− | +++ |
| % | 98.8% | 85% | 99.3% | |
| CB1 | ++ | + | +++/− | +++ |
| % | 98.8% | 79.5% | 98.6% | |
| CB2 | ++ | + | +++/− | +++ |
| % | 98.8% | 70.8% | 98.6% | |
| CB3 | ++ | – | +++/− | +++ |
| % | 97.7% | 4.97% | 97.9% | |
| CB4 | ++ | – | +++/− | +++ |
| % | 70.9% | 1.86% | 95.9% | |
| CB5 | ++ | – | +++/− | +++ |
| % | 72.7% | 1.86% | 92.5% | |
| Shape of elements | +++/− | + | +++/− | +++ |
| N | 172 | 161 | 147 | |
Embryos were assigned the following groups based on the severity of craniofacial malformations when compared to wild-type embryos;+++ wild-type, +++/− Mild malformation,++ Moderate malformation,+ Severe malformation, - Jaw elements absent or not stained.
Percentages reflect proportion of embryos with alcian blue stained cartilage elements to the total number of treated embryos expressed as a percentage.