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. Author manuscript; available in PMC: 2014 May 15.
Published in final edited form as: J Immunol. 2013 Apr 17;190(10):5086–5101. doi: 10.4049/jimmunol.1202071

FIGURE 3.

FIGURE 3

iNKT cells in T cell-specific Vps34-deficient mice. (A) Splenic, lymph node, thymic and liver mononuclear cells were prepared from Vps34f/f;CD4-Cre or Vps34f/+;CD4-Cre mice and were stained with anti-TCR-β, CD1d-tetramer and anti-B220 antibodies. (B) Frequency of iNKT cells in various lymphoid organs with 5–6 mice analyzed in each group from at least 3 independent experiments. *p<0.05. (C) Thymic iNKT cells were stained with CD1d-tetramer, and anti-CD8, -CD44 and -CD24 antibodies. Cells gated on tetramer+CD8α− cells are shown. For splenic iNKT cell analysis, B220+ cells were excluded from the analysis. A representative experiment of 3 independent experiments is shown.