Figure 3. Copy number of chimeric receptors in Jurkat cell lines evaluated by PCR.

The intensity of the PCR products from Jurkat cell lines expressing chimeric receptors was compared to standard copy number of parental Jurkat genomic DNA spiked with 1–16 copies of a CAR by (a) PCR and quantified using (b) Metamorph software. Shown is a representative experiment from 3 experiments performed.