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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2013 Mar 21;372(0):12–22. doi: 10.1016/j.mce.2013.03.004

Figure 1. c-Jun suppresses activities of multiple AR-responsive promoters.

Figure 1

(A) to (D) Effects of c-Jun overexpression on the activities of various androgen-responsive promoters in LNCaP cells. LNCaP cells were transfected with 0.5 μg of luciferase reporter driven by PSA promoter (A), MMTV promoter (B), probasin promoter (C), or synthetic ARE (D), 100 ng of pRL-TK and increasing concentrations (0, 10, 100, and 1000 ng) of pFlag-c-Jun expression vector. After 24 hr, cells were treated with ethanol (EtOH) or 10 nM R1881 for another 24 hr. (E) Western blot analyzing the AR and Flag-c-Jun expression in luciferase lysates from (A). (F) Effect of c-Jun overexpression on AR-transactivated PSA promoter in COS-1 cells. COS-1 cells were co-transfected with 1 μg of empty vector or pHA-AR, 0.5 μg PSA promoter, 100 ng of pRL-TK and increasing concentrations (0, 10, 100, and 1000 ng) of pFlag-c-Jun expression vector. After 24 hr, cells were treated with ethanol (vehicle control) or 10 nM R1881 for another 24 hr. Error bars, S.E. (n=3 for each experiment).