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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2013 Mar 21;372(0):12–22. doi: 10.1016/j.mce.2013.03.004

Figure 3. The transcriptionally inactive c-Jun mutants fail to efficiently suppress AR activity.

Figure 3

(A) Schematic view of various c-Jun mutants. NTD, N-terminal domain; LZ, leucine zipper, (B) and (C) Transcriptional activity of c-Jun mutants determined by luciferase reporters driven by the jun2 response element or three tandem repeats of TRE. LNCaP cells were transfected with 0.5 μg of jun2-luc (B) or 3XTRE-luc (C), 100 ng of pRL-TK along with 1 μg of pCMV-Flag (vector) or indicated pFlag-c-Jun (wild type or mutants) for 24 hr before assaying the luciferase activities. Error bars, S.E. (n=3). (D) Effects of c-Jun mutants on AR activity in LNCaP cells were evaluated by PSA reporter utilizing the method as described in legend of Figure 1. Error bars, S.E. (n=3~5). The asterisk and the double asterisk indicate P<0.05 and P<0.005, respectively (Student’s t-test). (E) Examination of the exogenous c-Jun expression in (D) by immunoblotting with a Flag antibody. (F) Effect of Flag-c-Jun mutants on endogenous PSA expression. LNCaP-rtTA-c-Jun cells (wild-type or mutants) were treated with 100 ng/ml Dox for 24 hr, followed by immunoblotting for AR, PSA and Flag-c-Jun proteins expression. All Flag-c-Jun mutants (Flag) were detected with the anti-Flag antibody.