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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2013 Mar 21;372(0):12–22. doi: 10.1016/j.mce.2013.03.004

Figure 4. Long-term c-Jun induction down-regulates AR expression at transcription level.

Figure 4

(A) LNCaP-rtTA-c-Jun or c-JunΔLZ cells were treated with 100 ng/ml doxycycline (Dox) for 72 hr, followed by immunoblotting analysis of AR, PSA and Flag-c-Jun expression. (B) and (C) LNCaP-rtTA-c-Jun or c-JunΔLZ cells were treated with Dox (100 ng/ml) for 24 hr. Transcripts of AR mRNA (B, n=4) and nascent AR RNA (C, n=4) were determined by qRT-PCR. The double asterisk indicates the P value between control (Con) and Dox groups is less than 0.01 (Student’s t-test). (D) LNCaP-rtTA-c-Jun cells were treated with Dox (100 ng/ml) for 24 hr followed by cycloheximide (10 μg/ml) treatment for indicated periods of time. AR and Flag-c-Jun were immunoblotted.